On that new item that appears around 0:17 -ish; does anyone else think it looks like some form of potion or bottle, With the blue circle part being the bottom of it? Since the trailer is blurred it's hard to tell, but it looks most like that to me.
Some other thoughts; it looks as if there's some kind of abandoned castle with forest growth in it (with the spider enemy), and the tight rope area looks very similar to the City of the Sky in TP. The pale guy at the end is certainly a cause for a lot of speculation; though I'm going to agree with what many people are saying that he's one of the interlopers.
Also, the music sounds really good, even in poor quality. If/when a better property version is released, I expect it'll sound even better, and if the rest of the music is this good, then that's great. The graphics look pretty polished as well and the new enemies look as if they require some more thought to defeat. Overall, I'm pretty excited now.