I am not entirely sure. If we do hear something about the Twili, I doubt it will be in a reference to the actual Twili in Twilight Princess. If there is a reference to the Twili, it will probably be the dark interlopers that started the Twili.
We learn in twilight princess that there was a "Dark Tribe" known to most as the "Dark Interlopers". They sought after the power of the Triforce and used the Fused Shadow to try and obtain the power of the Triforce. But they were stopped and sealed into the twilight realm through the mirror. Over time, they had children and evolved into the Twili.
If Skyward Sword has any reference to the Twili, it would be the Dark Interlopers. I can see Nintendo making a game with them as the bad guys as a giant back story to Twilight Princess and other games. I personally would like to see them in either Skyward Sword or even a future game. I feel that they deserve a better explanation and a game devoted to them.